Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans

Dawe, Adam S. and Nylund, Reeta and Leszczynski, Dariusz and Kuster, Niels and Reader, Tom and de Pomerai, David I. (2008) Continuous Wave and simulated GSM exposure at 1.8 W/kg and 1.8 GHz do not induce hsp16-1 heat-shock gene expression in Caenorhabditis elegans. Bioelectromagnetics, 29 (2). pp. 92-99. ISSN 1521-186X

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Abstract

Recent data suggest that there might be a subtle thermal explanation for the apparent induction by radiofrequency (RF) radiation of transgene expression from a small-heat-shock-protein (hsp16-1) promoter in the nematode, Caenorhabditis elegans. The RF fields used in the C. elegans study were much weaker (SAR 5-40 mW kg-1) than those routinely tested in many other published studies (SAR ~2 W kg-1). To resolve this disparity, we have exposed the same transgenic hsp16-1::lacZ strain of C. elegans (PC72) to higher intensity RF fields (1.8 GHz; SAR ~1.8 W kg-1). For both continuous wave (CW) and Talk-pulsed RF exposures (2.5 h at 25C), there was no indication that RF exposure could induce reporter expression above sham control levels. Thus, at much higher induced RF field strength (close to the maximum permitted exposure from a mobile telephone handset), this particular nematode heat-shock gene is not up-regulated. However, under conditions where background reporter expression was moderately elevated in the sham controls (perhaps as a result of some unknown co-stressor), we found some evidence that reporter expression may be reduced by ~15% following exposure to either Talk-pulsed or CW RF fields.

Item Type: Article
Additional Information: The definitive version is available at www3.interscience.wiley.com
Schools/Departments: University of Nottingham UK Campus > Faculty of Medicine and Health Sciences > School of Life Sciences
Depositing User: De-Pomerai, Dr David
Date Deposited: 14 Jun 2013 16:31
Last Modified: 13 Sep 2016 16:55
URI: http://eprints.nottingham.ac.uk/id/eprint/1979

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