Bacterial auto-nemesis: templating polymers for cell sequestration

Magennis, Eugene Peter (2013) Bacterial auto-nemesis: templating polymers for cell sequestration. PhD thesis, University of Nottingham.

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Abstract

The detection and control of microorganisms such as bacteria is important in a wide range of industries and clinical settings. Detection, binding and removal of such pathogenic contaminants can be achieved through judicious consideration of the targets which are available at or in the bacterial cell. Polymers have the ability to present a number of binding ligands for cell targeting on one macromolecule and so avidity of interaction can be greatly increased.

The goal of the project was to test whether polymers generated with bacteria in situ would have their composition significantly altered to determine if a templating process was occurring. It was also anticipated that the templated polymers would have better re-binding properties than those produced in the absence of bacteria.

A series of chemical functionalities were analysed for their binding properties to bacteria. The functionalities were chosen with consideration to the cell surface characteristics. Further to identification of the most binding and least binding functionalities the polymers were tested for their cytotoxicity against bacteria and human epithelial cells. Concentration ranges were determined which could facilitate bacterial binding and templating yet minimise the lethality of the processes.

Templated polymers of the bacteria were generated using a novel method of atom transfer radical polymerisation (ATRP) which we have termed bacterial activated atom transfer radical polymerisation (b-ATRP). This polymerisation method has maximised the potential for templating processes to occur during the polymerisation. Templated polymers differed in both their composition and their binding behaviour to non-templated polymers.

The bacterial organic reduction process has also been demonstrated to have greater scope for use within the organic chemistry field as demonstrated by the use of this system to enable in "click-chemistry" via the reduction of copper.

Item Type: Thesis (University of Nottingham only) (PhD)
Supervisors: Alexander, C.
Mantovani, G.
Subjects: Q Science > QR Microbiology > QR171 Microorganisms in the animal body
Q Science > QP Physiology > QP501 Animal biochemistry
Q Science > QR Microbiology > QR 75 Bacteria. Cyanobacteria
Faculties/Schools: UK Campuses > Faculty of Science > School of Pharmacy
Item ID: 14503
Depositing User: EP, Services
Date Deposited: 27 Aug 2014 12:10
Last Modified: 14 Sep 2016 22:37
URI: http://eprints.nottingham.ac.uk/id/eprint/14503

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