Analysis of the canonical initiation and trans-acting factor requirements of 5'TOP containing mRNAs
Garside, Paul (2010) Analysis of the canonical initiation and trans-acting factor requirements of 5'TOP containing mRNAs. PhD thesis, University of Nottingham.
All eukaryotic mRNAs process a cap structure (m7G(5')ppp(5')N) at the S' end of their message and most have an A as the first nucleotide after the cap. However, 30% of messages within eukaryotic cells have a C (m7G(t')ppp(5')C) as the first nucleotide followed by a short polypyrimidine tract. These mRNAs are termed TOP (Tenninal Oligopyrimidine tract) messages and are co-ordinately regulated by mitogenic, growth and nutritional stimuli. This work describes the construction of a reporter vector that encodes mRNA containing the TOP motif, and its use in a series of systematic experiments to further investigate the translational regulation of TOP messages. Given that TOP containing mRNAs are known to encode proteins involved in the translational machinery, these findings have important implications with regard to translational control and translation related disease.
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