A study of the fusion of higher plant protoplasts
Withers, Lyndsey A. (1973) A study of the fusion of higher plant protoplasts. PhD thesis, University of Nottingham.
Protoplasts and spontaneous fusion bodies can be isolated from a number of tissues, by treating the tissues with a mixture of macerating and cellulolytic enzymes. The shape of the spontaneous fusion bodies frequently reflects the parental tissue structure. An electron microscopic study of the treatment of the oat root tip and tobacco leaf with enzymes, reveals that spontaneous fusion is brought about by the expansion of plasmodesmatal connexions within the tissue. Specific treatments befo3B and during enzyme treatment can affect the level of spontaneous fusion.
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