Thermo-responsive surfaces for enzyme free mammalian cell culture
Dey, Sabrina (2010) Thermo-responsive surfaces for enzyme free mammalian cell culture. PhD thesis, University of Nottingham.
Embryonic stem cells are of great interest to scientists as they can differentiate into any somatic cell lineage making them excellent candidates for tissue regeneration and cell based treatment therapies. Currently, human embryonic stem cells (hESCs) are cultured using feeder fibroblasts or protein substrates such as matrigel, fibronectin or laminin in conditioned media. hESCs are then subcultured using enzymes to detach them from the culture substrate. However, the use of the xenosupport systems makes the hESCs therapeutic applications difficult due to cross-contamination with animal pathogens from the animal derived feeders, matrix or conditioned media to the hESCs. Moreover, the use of enzymes to recover hESCs can damage these cells. For the mentioned reasons, development of completely synthetic surfaces is desirable for hESCs culture.
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