Comparing yeast genome assemblies

Siow, Cheuk Chuen (2011) Comparing yeast genome assemblies. MRes thesis, University of Nottingham.

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The recent transition to Next-Generation Sequencing technology has accelerated the growth of genome projects exponentially. This explosion includes a multitude of species with different strains/individuals being sequenced and made available to the scientific community. As time passes, errors in genome assemblies are also being discovered and corrected. Biologists need to update their working assembly to a newer version or to convert between different strains or species for comparisons. The LiftOver utility in the UCSC Genome Browser handles these tasks with ease. Unfortunately, the choice for yeast genome conversions is limited. Here, I extend the capabilities of LiftOver by developing applications that generate the chain files required by LiftOver in an efficient way. These files are then utilised by a website that I built to allow conversion between assemblies, strains, or species of yeast using LiftOver. Also, I used R to produce dot-matrix plots of sequence alignment for rapid comparative analysis of a new genome sequence.

One important aspect of genome biology is the characterisation of the replication start sites, called DNA replication origin. Studies with confirmed and predicted replication origin locations, specifically in budding yeast Saccharomyces cerevisiae, are collated in a database (OriDB). However, the structure of OriDB is complex to maintain and currently includes just a description of S. cerevisiae replication origins. Here, I revamp the OriDB website and database to be future-proof so that additional studies or species can be added to the database without difficulties and maintenance can be carried out with ease. The database will also include data of Schizosaccharomyces pombe replication origins.

Item Type: Thesis (University of Nottingham only) (MRes)
Supervisors: Nieduszynski, C.
Subjects: Q Science > QH Natural history. Biology > QH426 Genetics
Faculties/Schools: UK Campuses > Faculty of Medicine and Health Sciences > School of Biology
Item ID: 12178
Depositing User: EP, Services
Date Deposited: 17 Oct 2012 12:34
Last Modified: 13 Sep 2016 12:04

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