Characterisation of Recombinant Aryl Hydrocarbon Receptor Ligand Binding Domain
Jiang, Tao (2004) Characterisation of Recombinant Aryl Hydrocarbon Receptor Ligand Binding Domain. PhD thesis, University of Nottingham.
Aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor, which mediates the toxicity of dioxin and related compounds, and has an important role in development. However, a structural basis for ligand binding to the AhR remains unclear and the study was hindered by the low abundance and inherited instability of the AhR. Based on a previously defined minimal ligand-binding domain (LBD, residues 230-421), in the present study a series of truncated LBD constructs were created and expressed in insect cells (Sf9) using a baculovirus expression system.
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